Increased CD34 + /KDR + cells are not associated with carotid artery intima-media thickness progression in chronic HIV-positive subjects
Year: 2011
Type: article
Abstract: BACKGROUND: Endothelial progenitor cells (EPCs) are involved in the endothelium repair. Low circulating EPC levels are predictive of cardiovascular events in HIV-negative subjects. The impact of HIV infection on EPCs, and the role of EPCs in HIV-associated cardiovascular disease, is not known. We hypothesized that circulating EPCs would be inversely associated with carotid artery intima-media thickness (c-IMT) changes in HIV-infected subjects. METHODS: EPCs (CD34(+)/KDR(+), CD133(+)/KDR(+) and CD34(+)/CD133(+)/KDR(+)) were defined retrospectively by flow cytometry in cryopreserved peripheral blood mononuclear cells collected longitudinally from 66 chronic HIV-infected subjects and cross-sectionally from 50 at-risk HIV-negative subjects. The HIV-infected subjects participated in the Study of the Consequences of the Protease Inhibitor Era (SCOPE) cohort, were receiving antiretroviral therapy (59/66) and had two sequential measurements of c-IMT 1 year apart. Two distinct groups of HIV-infected subjects were identified a priori: rapid c-IMT progressors (subjects with rapid c-IMT progression, n=13, Δc-IMT>0.2 mm) and slow c-IMT progressors (subjects with slow or no c-IMT progression, n=53, Δc-IMT<0.2 mm). RESULTS: Although cryopreservation reduced sensitivity of detection, EPC frequency in HIV-infected subjects was still significantly higher compared to at-risk HIV-negative subjects (CD34(+)/KDR(+); P=0.01) and correlated positively with CD4(+) T-cell count (CD34(+)/KDR(+), r=0.27; P=0.03). No association was found between the change of EPC frequencies over time (ΔEPC) and Δc-IMT or between EPC frequencies and c-IMT or Δc-IMT. CONCLUSIONS: The lack of an association between EPCs and c-IMT in our cohort does not support HIV-associated reductions in EPC frequency as a cause of accelerated atherosclerosis.
Source: Antiviral Therapy
DOI: 10.3851/imp2013
Language: English
Subfield: Emergency Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: Pennsylvania Department of Health, National Institutes of Health, Center for AIDS Research, University of Washington, University of California, San Francisco, National Heart, Lung, and Blood Institute, National Institute of Mental Health, National Institute of Allergy and Infectious Diseases, National Center for Research Resources
Awards: G1572886882, Clinical and Translational Science Institute, G1717064709, Impact of HIV-associated inflammation on disease, UCSF-GIVI CENTER FOR AIDS RESEARCH, G200995756, G2152088972, HIV Therapy & Interruption RCT in Resource Poor Clinic, G330126873, G4003025817, G4610316350, G489831256, G5418282550, G6257732833, G6702432869, IMMUNE AND VIRAL OUTCOMES OF HIV-1 THERAPY INTERRUPTION, G81379742, Center for AIDS Prevention Studies (CAPS), G955205799, G97119092
OpenAlex ID: W2044876820