Work

Microscopy refocusing and dark-field imaging by using a simple LED array

Year: 2011

Type: article

Abstract: The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

Source: Optics Letters

DOI: 10.1364/ol.36.003987

Authors: Guoan Zheng, Christopher Kolner, Changhuei Yang

Institution: California Institute of Technology

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: bronze

Funder: U.S. Department of Defense

Award: INTEGRATED DEVICE FOR CIRCULATING TUMOR CELL CAPTURE, CHARACTERIZATION, AND LENS-FREE MICROSCOPY