Work

Appearance of an arachidonic acid 15‐lipoxygenase pathway upon differentiation of the human promyelocytic cell‐line HL‐60

Year: 1985

Type: article

Abstract: The metabolism of arachidonic acid and 15-HPETE was studied in a human promyelocytic cell line (HL-60). Upon exposure to DMSO, HL-60 cells undergo differentiation and acquire a 15-lipoxygenase activity while undifferentiated cells challenged with either arachidonic acid or 15-HPETE did not enzymatically transform these precursors. Products of the arachidonic acid 15-lipoxygenase pathway were identified by HPLC. UV-absorption and gas chromatography-mass spectrometry. Results indicate that upon differentiation HL-60 cells express a 15-lipoxygenase activity as well as the ability to transform 15-HPETE to 8,15-DHETEs and 14,15-DHETE. Moreover, these findings suggest that products of the 15-lipoxygenase cascade may be generated by a single cell system.

Source: FEBS Letters

DOI: 10.1016/0014-5793(85)80731-6

Authors: Ulf Lundberg, Charles N. Serhan, Bengt Samuelsson

Institution: Karolinska Institutet

Language: English

Topic: Eicosanoids and Hypertension Pharmacology

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Arthritis Foundation, Vetenskapsrådet, Medical Research Council, National Cancer Institute