Work

Direct physical interaction involving CD40 ligand on T cells and CD40 on B cells is required to propagate MMTV

Year: 1995

Type: article

Abstract: The propagation of mouse mammary tumor virus (MMTV) has been analyzed in mice defective for expression of CD40 ligand (CD40L). Mice with endogenous viral superantigen (SAG) delete T cells with cognate V beta independent of CD40L expression. Nevertheless, CD40L-mice do not show deletion of cognate T cells after being exposed to infectious MMTV and have greatly diminished viral replication. The response of CD40L- T cells to SAG in vitro is also impaired, but can be reconstituted by adding B cells activated by recombinant CD40L to express costimulatory molecules. Thus, direct CD40L-dependent B cell activation appears to be a critical step in the life cycle of MMTV. The initial step in SAG-dependent T cell activation, and hence the MMTV life cycle, may be mediated by non-B cells, because splenocytes from B cell-deficient SAG-transgenic mice are able to activate cognate T cells.

Source: Immunity

DOI: 10.1016/1074-7613(95)90166-3

Authors: Alexander V. Chervonsky, Jianchao Xu, Avlin K. Barlow, Marilyn Khery, Richard A. Flavell, Charles A. Janeway

Institutions: Howard Hughes Medical Institute, Yale University, Boehringer Ingelheim (United States)

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $10,400