Work

Defined DNA/nanoparticle conjugates

Year: 2005

Type: article

Abstract: Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.0506290102

Authors: Christopher J. Ackerson, Michael T. Sykes, Roger D. Kornberg

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases