Work

Photochemical DNA Activation

Year: 2007

Type: article

Abstract: A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.

Source: Organic Letters

DOI: 10.1021/ol070455u

Authors: Hrvoje Lusic, Douglas D. Young, Mark O. Lively, Alexander Deiters

Institutions: North Carolina State University, Wake Forest University Health Sciences, Wake Forest University

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: G1674938525, G424083508