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Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

Year: 2011
Type: article
Abstract: The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH(2) stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.
DOI: 10.1117/1.3533716
Authors: Chia-Yu Lin, Eric O. Potma, Jeffrey L. Suhalim, Chyong Ly Nien, James V. Jester, Miloš D. Miljkovic, Max Diem
Language: English
Subfield: Biophysics
Open Access status: bronze
Funders: National Science Foundation, Research to Prevent Blindness, National Institutes of Health, University of California, Irvine, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources
Awards: G1159671548, G2326367487, IDBR: A Focus-Engineered Coherent Anti-Stokes Raman Scattering (FE-CARS) Microscope for Biological Imaging, A Laser Microbeam Biotechnology Resource, Mathematical, Computational and Systems Biology, G8565522207, G9000199918
OpenAlex ID: W2046066571
FWCI: 6.088
Cites: 42
Cited by: 79
Related to: 10