Year: 2008
Type: article
Abstract: Recombinant Omp28 (r-Omp28) from Brucella melitensis produced in Escherichia coli was previously identified as group 3 proteins. In this study, we evaluated the immunogenicity of r-Omp28 in mice. This r-Omp28 was injected intramuscularly in the mice with and without CpG oligodeoxynucleotides (ODN). The booster was given on 3rd week with the same antigenic preparation. Specific antibodies to purified r-Omp28 were detected in mice sera by western blotting and indirect ELISA (iELISA). In addition, isotype (IgG1 and IgG2a) specific antibodies were also measured by iELISA. Cellular immune response was measured by lymphocyte proliferation assay (MTT assay) after in vitro stimulation of spleenocytes by r-Omp28. Immunization with r-Omp28 induced a vigorous immunoglobulin G (IgG) response, with higher IgG1 than IgG2a. Whereas, immunization of mice with rOmp28 + CpG induced IgG2a dominated response, suggesting the induction of a T helper 1 (Th1) response by CpG ODN. Spleenocyte from immunized mice showed significant proliferative response (3 week post booster), but it was higher (P < 0.05) in r-Omp28 + CpG than r-Omp28 immunized mice. Our results indicate that r-Omp28 is a good immunogen, capable of inducing both humoral and cellular immune response. The humoral response was biased towards Th1 type when it was co-administered with CpG ODN.
Authors: P. Kaushik, P. Chaudhury, G. Shukla, D.K. Singh
Institution: Indian Veterinary Research Institute
Language: English
Subfield: Small Animals
Field: Veterinary
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,900
OpenAlex ID: W2046238641