Year: 2013
Type: article
Abstract: Enteropeptidase can cleave trypsinogen on the sequence of Asp-Asp-Asp-Asp-Lys and plays an important role in food digestion. The RANKL-RANK signalling pathway plays a pivotal role in bone remodelling. In this study, we reported that enteropeptidase can inhibit the RANKL-RANK signalling pathway through the cleavage of RANK. A surrogate peptide blocking assay indicated that enteropeptidase could specifically cleave RANK on the sequence NEEDK. Osteoclast differentiation assay and NF-κB activity assay confirmed that enteropeptidase could inhibit osteoclastogenesis in vitro through the cleavage of RANK. This is the first study to prove that the RANKL-RANK signalling pathway can be inhibited by cleavage of RANK instead of targeting RANKL.
Source: FEBS Letters
Authors: Yunfeng Zhao, Mengmeng Jin, Juan Ma, Shiqian Zhang, Wei Li, Yuan Chen, Yingsheng Zhou, Hong Tao, Yu Liu, Lei Wang, Huamin Han, Ge Niu, Hua Tao, Changzhen Liu, Bin Gao
Language: English
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2046289627