Work

Facilitated Assignment of Adenine H2 Resonances in Oligonucleotides Using Homonuclear Long-Range Couplings

Year: 2009

Type: article

Abstract: The small homonuclear coupling constant between H2 and H8 protons of adenine (0.5 +/- 0.2 Hz) was determined and exploited to assign the H2 proton of adenine residues in DNA oligonucleotides. The utility of this approach is demonstrated for two different DNA sequences in D(2)O, for which we show a rapid and unambiguous assignment of the A H2 resonances.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja900771b

Authors: Jin Zhang, Alexander Spring, Markus W. Germann

Institution: Georgia State University

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: G1901347875, NMR STRUCTURE OF ZINC FINGERS SPECIFIC FOR HIV RRE RNA