Work

Laser microbeam abalation of GFP-labeled nuclear organelles in a living cell.

Year: 1997

Type: conference-paper

Abstract: Cancer, development, cellular growth and differentiation are governed by gene expression. Recent molecular and cellular advances to visualize and perturb the pathways of transcriptional regulation, nascent RNA processing, and protein trafficking at the single cell level have been developed. More recently, applications utilizing the green fluorescent marker (GFP) from Aequorea victoria have facilitated visualization of these molecular events in a living cell. Specifically, we will describe a novel approach to perturb cellular processes by labeling discrete cellular components of interest with GFP and subsequently altering/ablating them with a laser microbeam.

Source: Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE

DOI: 10.1117/12.274333

Authors: Vickie J. LaMorte, Tatiana B. Krasieva, Ronald M. Evans, Michael W. Berns, Bruce J. Tromberg

Institutions: Beckman Laser Institute and Medical Clinic, Howard Hughes Medical Institute

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: American Cancer Society, National Institutes of Health, National Center for Research Resources

Award: G9000088035