Year: 2013
Type: article
Abstract: Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica.
Source: PLoS ONE
Institution: University of Vermont
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: G1110558534, Vermont Immunobioloigy/ Infectious Diseases Center, G7198170567, Molecular Mechanism of Entamoeba histolytica phagocytosis, G8021174655, G8339823181, Molecular regulation of HIV-1 assembly, release and cell-to-cell transmission, Vermont Immunology / Infectious Diseases Training Grant
OpenAlex ID: W2046877441