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Application of Ultrasonic Treatment on Collecting Recombinant Protein from Yeast

Year: 2014

Type: article

Abstract: An appropriate ultrasonication of collecting foreign recombinant protein (human-adiponectin) from yeast was established. Regarded the bioactivity of foreign recombinant protein tested by Western Blotting analysis as parameter, a L9(33) orthogonal array design based on a series of single-factor experiments was employed to optimize conditions for ultrasonic disruption. The results showed that the ultrasonic power of 450 W, duration time on 25 min and operation interval (work time: intermittent time) of 10:10 (s/s) were optimal process with the highest protein bioactivity, and meanwhile, the cell breaking rate was (67.8±2.1) %.

Source: Advanced materials research

DOI: 10.4028/www.scientific.net/amr.998-999.191

Authors: Jia Kun Dai, Yan Li, Hui Hui Zhuang, Xi Chen, Qi Ma, Sheng Yun Xu, Tao Qin, Ya Hui Wei

Institutions: Shaanxi Academy of Sciences, Microbiology Institute of Shaanxi, Northwest University

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W2046942937

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Cites: 13

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Related to: 10