Work

1050 Applicability of perfusion MRI in monitoring cell-based therapy in a rabbit hindlimb ischemia model

Year: 2008

Type: article

Abstract: At baseline, the difference in MR perfusion upslope time between ischemic (I) and non-ischemic (NI) limbs was worse in MSC-treated rabbits (5.0 ± 3 s) vs. controls (3.7 ± 3 s). At 2 weeks, TIMI frame counts showed similar therapeutic effect in MSC and sham groups (7.7 ± 3 vs. 7.3 ± 3 s, P = NS). However, MRI perfusion in MSC rabbits showed more improvement (difference decreased 2.1 ± 1 s, P < 0.04 vs. baseline) than controls (1.0 ± 1 s improvement, P = NS), which mirrored histological analysis results. Increased capillary density in the left limb relative to the right limb based on histological analysis (28 ± 20 MSC vs. 19 ± 22 control difference I vs. N, P = NS) was present in all animals except one control animal. Furthermore, histological analysis confirms incorporation of the MSCs into the vessel walls. In this preclinical model, MSC therapy show modest improvements in MRI perfusion relative to controls. MRI offers a non-invasive means to serially determine tissue perfusion without arterial access that is concordant with histological gold standards.

Source: Journal of Cardiovascular Magnetic Resonance

DOI: 10.1186/1532-429x-10-s1-a175