Work

siRNA Selection Server: an automated siRNA oligonucleotide prediction server

Year: 2004

Type: article

Abstract: The Whitehead siRNA (short interfering RNA) Selection Web Server (http://jura.wi.mit.edu/bioc/siRNA) automates the design of short oligonucleotides that can specifically 'knock down' expression of target genes. These short sequences are about 21 nt in length, and when synthesized as double stranded RNA and introduced into cell culture, can reduce or eliminate the function of the target gene. Depending on the length of a gene, there are potentially numerous combinations of possible 21mers. Some experimental evidence has already shown that not all 21mers in a gene have the same effectiveness at silencing gene function. Our tool incorporates published design rules and presents the scientist with information about uniqueness of the 21mers within the genome, thermodynamic stability of the double stranded RNA duplex, GC content, presence of SNPs and other features that may contribute to the effectiveness of a siRNA.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkh366

Authors: B. Yuan, R. Latek, M. Hossbach, T. Tuschl, F. Lewitter

Institutions: Whitehead Institute for Biomedical Research, Max Planck Society, Max Planck Institute for Biophysical Chemistry, Rockefeller University

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,992

OpenAlex ID: W2047277267