Work

Expression of Type X collagen mRNA levels in embryonic chick sternum during development

Year: 1986

Type: article

Abstract: Embryonic chick sternum cartilage exhibits profound spatial and temporal changes in Type X collagen biosynthesis during development. Production of this collagen is confined to the presumptive calcification region and its expression is not acquired until stage 43. To examine the mechanisms responsible for regulation of developmental changes in biosynthetic expression of Type X collagen, we determined the levels of translatable Type X procollagen mRNA employing a cell-free translation system. We found that mRNA capable of directing Type X collagen synthesis was present exclusively in cartilage destined to undergo calcification and that its levels were nearly equivalent at all stages of development. These findings suggest that expression of Type X collagen in embryonic chick sternum is determined at the translational level.

Source: Biochemical and Biophysical Research Communications

DOI: 10.1016/0006-291x(86)90342-6

Authors: Anthony M. Reginato, James W. Lash, Sergio A. Jimenez

Institution: University of Pennsylvania

Language: English

Topic: Osteoarthritis Treatment and Mechanisms

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,110

Funder: National Institutes of Health