Work

Characterisation of γ‐glutamylcysteine synthethase‐heavy subunit promoter: a critical role for AP‐1

Year: 1998

Type: article

Abstract: The 5'-flanking region of human gamma-glutamylcysteine synthetase-heavy subunit (gamma-GCS-HS) was characterised by creating a series of chloramphenicol acetyl transferase (CAT) reporter deletion constructs. Analysis of various deleted CAT constructs revealed that a putative AP-1 consensus sequence is required to direct the constitutive and oxidant-mediated promoter activity. Gel mobility shift and mutation analysis of the sequence (-269 to -263 bp), showed binding of AP-1 is involved in the oxidant-mediated regulation of gamma-GCS-HS promoter activity.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(98)00410-4

Authors: Irfan Rahman, Christopher A.D Smith, Frank Antonicelli, William MacNee

Institutions: Edinburgh Royal Infirmary, University of Edinburgh

Language: English

Topic: Genomics, phytochemicals, and oxidative stress

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: British Lung Foundation

OpenAlex ID: W2047873700

FWCI: 2.557

Cites: 21

Cited by: 72

Related to: 10