Work

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Year: 2011

Type: article

Abstract: Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.

Source: IEEE Journal of Selected Topics in Quantum Electronics

DOI: 10.1109/jstqe.2011.2168559

Authors: Yuan Liu, Haohua Tu, Wladimir A. Benalcazar, Eric J. Chaney, Stephen A. Boppart

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Laser-Matter Interactions and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: G178577521, G3210179440, Nonlinear Interferometric Vibrational Imaging of Cancer, Nonlinear Interferometric Vibrational Imaging of Cancer