Work

Generation and characterization of human monoclonal antibodies to G5, a linear neutralization epitope on glycoprotein of rabies virus, by phage display technology

Year: 2008

Type: article

Abstract: The aim of the present study was to discover distinct human MAbs to RV with high neutralizing potency and a broad neutralization spectrum. A phage display technology was used to produce human scFv to G5, a conserved linear neutralization epitope on Gp of RV. A phage display scFv library with 6 x 10(7) members was constructed and the phage-scFv with 'antigen-binding' activities were selected with synthetic peptide G5-24. The obtained scFv genes were cloned into pET22b(+)/BL21(DE3) and from this we prepared purified scFv fragments. The assay of the specificity characteristics and neutralization capacity showed that two distinct clones with new human immunoglobulin V genes can recognize G5 specifically as well as neutralize different RV strains. They have potential for inclusion in an antibodies combination aimed for use in rabies PEP.

Source: Microbiology and Immunology

DOI: 10.1111/j.1348-0421.2008.00016.x

Authors: Xiao‐Ling Zhao, Jun Yin, Wei‐Qiang Chen, Ming Jiang, Guan Yang, Zhi‐Hua Yang

Institutions: Academy of Military Medical Sciences, National Center of Biomedical Analysis, Institute of Microbiology, Beijing Institute of Microbiology and Epidemiology

Language: English

Topic: Rabies epidemiology and control

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2048254480

FWCI: 0.673

Cites: 20

Cited by: 25

Related to: 10