Work

Novel Designs for Centrifugal Countercurrent Chromatography: V. Comparative Studies on Performance of Various Column Configurations

Year: 2010

Type: article

Abstract: The conventional toroidal coil in centrifugal countercurrent chromatography has a low level of stationary phase retention, since a half of each helical turn is entirely occupied by the mobile phase. In order to cope with this problem, several new column designs including zigzag, saw-tooth and figure-8 patterns have been introduced and their performance was compared in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate number (N) and column pressures. Overall results of experiments indicate that the figure-8 column yields the highest Rs when the lower phase is used as the mobile phase. Since the column pressure of all these new columns are much lower than that in the traditional toroidal coil column, the separation efficiency can be improved using a long separation column without a risk of column damage by high back pressure.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826076.2010.503780

Authors: Yi Yang, Dongyu Gu, Haji Akber Aisa, Yoichiro Ito

Institutions: National Institutes of Health, Chinese Academy of Sciences, Xinjiang Technical Institute of Physics & Chemistry, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute

Award: Novel column design for centrifugal countercurrent chromatography