Work

Traceable clonal culture and chemodrug assay of heterogeneous prostate carcinoma PC3 cells in microfluidic single cell array chips

Year: 2014

Type: article

Abstract: Cancer heterogeneity has received considerable attention for its role in tumor initiation and progression, and its implication for diagnostics and therapeutics in the clinic. To facilitate a cellular heterogeneity study in a low cost and highly efficient manner, we present a microfluidic platform that allows traceable clonal culture and characterization. The platform captures single cells into a microwell array and cultures them for clonal expansion, subsequently allowing on-chip characterization of clonal phenotype and response against drug treatments. Using a heterogeneous prostate cancer model, the PC3 cell line, we verified our prototype, identifying three different sub-phenotypes and correlating their clonal drug responsiveness to cell phenotype.

Source: Biomicrofluidics

DOI: 10.1063/1.4900823

Authors: Jaehoon Chung, Patrick N. Ingram, Tom Bersano-Begey, Euisik Yoon

Institution: University of Michigan

Language: English

Topic: Microfluidic and Bio-sensing Technologies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Science Foundation, Korea Institute of Science and Technology, National Heart, Lung, and Blood Institute, National Cancer Institute