Work

Linking Lysosomal Trafficking Defects with Changes in Aging and Stress Response inDrosophila

Year: 2007

Type: article

Abstract: Defects in pathways that direct cellular components to the lysosome for degradation are often linked with a decrease in viability and with progressive disorders. Previously we had shown that blue cheese (bchs: Drosophila homologue of human Alfy) mutations lead to reduced longevity and the accumulation of ubiquitinated neural aggregates. A genetic modifier screen based on overexpression of Bchs in the eye was used to identify several potential genetic interactions, which included autophagic and endocytic trafficking genes as well as cytoskeletal and motor proteins and members of the SUMO and ubiquitin signaling pathways. We found that mutations in several of the genes identified in the screen also result in bchs-like phenotypes, including a reduction in adult lifespan and changes in ubiquitinated protein profiles. In addition, we show that Bchs modifiers belonging to the autophagic and trans-Golgi trafficking pathways also display defects in adult starvation response. Our data further support a role for Bchs/Alfy in the autophagic pathway and strongly indicate that autophagy plays an important role in aging and stress response.Addendum to:Genetic Modifiers of the Drosophila Blue Cheese Gene Link Defects in Lysosomal Transport with Decreased Lifespan and Altered Ubiquitinated Protein ProfilesA. Simonsen, R. Cumming, K. Lindmo, V. Galaviz, S. Cheng, T. Rusten and K. FinleyGenetics 2007; In press

Source: Autophagy

DOI: 10.4161/auto.4604

Authors: Anne Simonsen, Robert C. Cumming, Kim D. Finley

Institution: Salk Institute for Biological Studies

Language: English

Topic: Autophagy in Disease and Therapy

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Norges Forskningsråd, National Institutes of Health