Work

Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.

Year: 1990

Type: article

Abstract: Catabolite repression of the Bacillus subtilis alpha-amylase gene (amyE) involves an operator sequence located just downstream of the promoter (amyR), overlapping the transcription start site. Oligonucleotide site-directed mutagenesis of this sequence identified bases required for catabolite repression. Two mutations increased both the 2-fold symmetry of the operator and the repression ratio. Although many mutations reduced the repression ratio 3- to 11-fold, some also caused a 2-fold or greater increase in amylase production. Others caused hyperproduction without affecting catabolite repression. Homologous sequences in other catabolite-repressed B. subtilis promoters suggest a common regulatory site may be involved in catabolite repression.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.87.16.6238

Authors: M J Weickert, G H Chambliss

Institution: University of Wisconsin–Madison

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, U.S. Public Health Service, National Institute of General Medical Sciences

Awards: GENETIC ANALYSIS OF BACILLUS SUBTILIS AMYLASE SYNTHESIS, Predoctoral Training Program in Genetics