Work

Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Simultaneous Detection of Stimulants and Diuretics in Urine

Year: 2011

Type: article

Abstract: This study established a simultaneous screening method based on solid-phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS-MS) for the detection of 23 stimulants and 23 diuretics in human urine. An electrospray ionization source and multiple reaction monitoring were used for data acquisition. All stimulants and diuretics were separated in less than 12.52 min. The limits of detection were in the range of 25-500 ng/mL for stimulants and 25-125 ng/mL for diuretics. To evaluate the performance of this method, urine samples were collected from 1627 athletes in Taiwan, and 7 positive samples were found. This LC-MS-MS method not only meets the minimum required performance limits set by the World Anti-Doping Agency but also provides a fast way to analyze the authentic urine samples in doping control laboratories.

Source: Journal of Analytical Toxicology

DOI: 10.1093/anatox/35.9.665

Authors: Ku Fu Hsu, Kuei-Yu Chien, Guo Ping Chang-Chien, Su Fan Lin, Pei Hsuan Hsu, Mei-Chich Hsu

Institutions: National Taiwan Sport University, Kainan University, Cheng Shiu University

Language: English

Topic: Doping in Sports

Subfield: Sociology and Political Science

Field: Social Sciences

Domain: Social Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: National Science Council