Work

High‐efficiency cloning of Arabidopsis full‐length cDNA by biotinylated CAP trapper

Year: 1998

Type: article

Abstract: Full-length cDNAs are essential for functional analysis of plant genes. We constructed high-content, full-length cDNA libraries from Arabidopsis thaliana plants based on chemical introduction of a biotin group into the diol residue of the CAP structure of eukaryotic mRNA, followed by RNase I treatment, to select full-length cDNA. More than 90% of the total clones obtained were of full length; recombinant clones were obtained with high efficiency (2.2 x 10(6)/9 micrograms starting mRNA). Sequence analysis of 111 randomly picked clones indicated that 32 isolated cDNA groups were derived from novel genes in the A. thaliana genome.

Source: The Plant Journal

DOI: 10.1046/j.1365-313x.1998.00237.x

Authors: Motoaki Seki, Piero Carninci, Yoko Nishiyama, Yoshihide Hayashizaki, Kazuo Shinozaki

Institutions: RIKEN, Japan Science and Technology Agency

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funders: Japan Science and Technology Corporation, Japan Science and Technology Agency, RIKEN, Core Research for Evolutional Science and Technology