Work

A Selective, Cell-Permeable Optical Probe for Hydrogen Peroxide in Living Cells

Year: 2004

Type: article

Abstract: We present the synthesis, properties, and biological applications of Peroxyfluor-1 (PF1), a new type of optical probe for intracellular imaging of hydrogen peroxide in living biological samples. PF1 utilizes a boronate deprotection mechanism to provide unprecedented selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical. We further demonstrate the value of this reagent for biological applications by imaging changes in [H2O2] in living mammalian cells.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja0441716

Authors: Michelle C. Y. Chang, Arnd Pralle, Ehud Y. Isacoff, Christopher J. Chang

Institution: University of California, Berkeley

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of Neurological Disorders and Stroke

Award: Fluorescent Protein Sensors of Neural Activity