“On-the-Fly” Kinetics of Enzymatic Racemization Using Deuterium NMR in DNA-Based Chiral Oriented Media
Year: 2013
Type: article
Abstract: We report the in situ and real-time monitoring of the interconversion of L- and D-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by (2)H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their (2)H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.
Source: Analytical Chemistry
DOI: 10.1021/ac4004002
Institutions: Centre National de la Recherche Scientifique, Inserm, École Normale Supérieure - PSL, Laboratoire des Biomolécules, Université Pierre-et-Marie-Curie, Institut Curie, Université Paris-Sud, Institut de Chimie Moléculaire et des Matériaux d'Orsay, École Polytechnique Fédérale de Lausanne, University of California, Davis, Indian Institute of Science Bangalore
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funder: Agence Nationale de la Recherche
OpenAlex ID: W2050214137