Work

Synthesis of DNA via deoxynudeoside H-phosphonate Intermediates

Year: 1986

Type: article

Abstract: Deoxynucleoside H-phosphonates are used in the chemical synthesis of deoxyoligonucleotides up to 107 bases in length. The biological activity of the synthetic DNA is assessed by cloning into M13 and sequencing. An improved synthesis of protected deoxynucleoside H-phosphonates is also described.

Source: Nucleic Acids Research

DOI: 10.1093/nar/14.13.5399

Authors: Brian C. Froehler, Peter G. Ng, Mark D. Matteucci

Institution: Genentech

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2050289195

FWCI: 5.509

Cites: 4

Cited by: 349

Related to: 9