Work

Novel Methylotrophy Genes of Methylobacterium extorquens AM1 Identified by using Transposon Mutagenesis Including a Putative Dihydromethanopterin Reductase

Year: 2003

Type: article

Abstract: Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR). This mutant has a C(1)-defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (H(4)MPT)-dependent formaldehyde oxidation. These results suggest that this gene, dmrA, may encode dihydromethanopterin reductase, an activity analogous to that of DHFR that is required for the final step of H(4)MPT biosynthesis.

Source: Journal of Bacteriology

DOI: 10.1128/jb.185.2.669-673.2003

Authors: Christopher J. Marx, Brooke N. O'Brien, Jennifer Breezee, Mary E. Lidstrom

Institution: University of Washington

Language: English

Topic: Microbial metabolism and enzyme function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Integrated Metabolism in Methylobacterium extroquens AM1, G2608117561