Work

Na+/Ca2+ exchange in isolated smooth muscle cells de monstrated by the fluorescent calcium indicator fura‐2

Year: 1986

Type: article

Abstract: Fura-2, a novel fluorescent indicator of cytoplasmic calcium concentrations ([Ca2+i]), was 'loaded' into smooth muscle cells isolated from guinea pig taenia coli. Resting cells maintained a stable [Ca2+i] of 107 +/- 26 nM (n = 13), which could be perturbed with ionomycin. [Ca2+i] was elevated by stimulation of the cells with carbachol or 50 mM KCl. Reduction of the plasmalemmal Na+ concentration gradient by inhibition of the Na+/K+-ATPase with ouabain markedly elevated [Ca2+i]; this elevation was dependent on extracellular Ca2+. [Ca2+i] was also increased by replacement of the extracellular Na+ with an organic cation.

Source: FEBS Letters

DOI: 10.1016/0014-5793(86)80122-3

Authors: K. Pritchard, C.C. Ashley

Institution: University of Oxford

Language: English

Topic: Ion channel regulation and function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Medical Research Council

OpenAlex ID: W2050730220

FWCI: 3.164

Cites: 11

Cited by: 33

Related to: 10