Work

K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells.

Year: 1989

Type: article

Abstract: Acidification of the cytoplasm of Ehrlich ascites carcinoma cells to pH 6.3 arrests DNA synthesis in these cells. Such an effect can be achieved by incubating the cells at pH 6.2 or by adding low concentrations of the K+/H+ antiporter, the antibiotic nigericin, at neutral pH. Glucose and anaerobiosis potentiate the nigericin effect. The inhibition of DNA synthesis by nigericin occurs without any significant decrease in the ATP concentration and in the mitochondrial membrane potential. The DNA synthesis inhibition is caused neither by a decrease in the intracellular [K+] nor by an increase in the intracellular [Na+] accompanying the nigericin effect (at least at low concentrations of the antibiotic). Nigericin should thus be regarded as a type of a cytostatic primarily affecting intracellular pH.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.86.17.6626

Authors: L B Margolis, Y u Novikova I, I A Rozovskaya, V P Skulachev

Institution: Lomonosov Moscow State University

Language: English

Topic: Ion Transport and Channel Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2050786791

FWCI: 0.516

Cites: 12

Cited by: 27

Related to: 10