Work

Tissue Factor Activity in Lymphocyte Cultures from Normal Individuals and Patients with Hemophilia A

Year: 1973

Type: article

Abstract: The procoagulant material of lymphocytes has been characterized as tissue factor. Lymphocytes stimulated with phytohemagglutinin or the purified protein derivative of the tubercle bacillus developed procoagulant activity with incubation in tissue culture. While this material corrected the prolonged clotting time of factor VIII (AHF) deficient plasma, we have shown, utilizing a sensitive radioimmunoassay, that no AHF antigen was present in the cell cultures. Further, we have demonstrated this material to be tissue factor by coagulation techniques and immunological cross-reactivity. The published data regarding factor VIII synthesis is reviewed in light of these observations and comments are made regarding the role of the lymphocyte procoagulant.

Source: Journal of Clinical Investigation

DOI: 10.1172/jci107316

Authors: Frederick R. Rickles, John A. Hardin, Frances A. Pitlick, Leon W. Hoyer, Marcel E. Conrad

Institutions: National Institutes of Health, United States Department of Veterans Affairs, University of Connecticut, Walter Reed National Military Medical Center, Yale University, UConn Health, Walter Reed Army Institute of Research

Language: English

Topic: Hemophilia Treatment and Research

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2050923504

FWCI: 8.997

Cites: 47

Cited by: 110

Related to: 10