Work

Receptor‐independent metabolism of platelet‐activating factor by myelogenous cells

Year: 1989

Type: article

Abstract: Human neutrophils incorporate and metabolize platelet-activating factor (PAF). We dissociated these events from PAF binding to its receptors. Cells were pretreated with either pronase, a PAF antagonist (L652731), or excess PAF. This reduced PAF receptor numbers by 70 to almost 100% but had no comparable effect upon the neutrophil's ability to metabolize PAF. Furthermore, HL-60 cells efficiently metabolized, but did not specifically bind, PAF. Thus, PAF receptor availability did not correlate with PAF metabolic capacity and we conclude that myelogenous tissues can process this bioactive ligand by a receptor-independent pathway.

Source: FEBS Letters

DOI: 10.1016/0014-5793(89)80751-3

Authors: Joseph T. O'Flaherty, Marie C. Chabot, Jimmy Redman, David Jacobson, Robert L. Wykle

Institutions: Atrium Health Wake Forest Baptist, Wake Forest University Health Sciences, Wake Forest University

Language: English

Topic: Sphingolipid Metabolism and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute