Work

A Selective Turn-On Fluorescent Sensor for Imaging Copper in Living Cells

Year: 2005

Type: article

Abstract: We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja055064u

Authors: Li Zeng, Evan W. Miller, Arnd Pralle, Ehud Y. Isacoff, Christopher J. Chang

Institution: University of California, Berkeley

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke

Awards: University of California Berkeley Chemistry-Biology Interface Training Program, Structural Rearrangements in Channel Gating