Year: 2014
Type: article
Study design: Observational Study
Abstract: Background: Ethiopia is one of the countries with high incidence and prevalence of TB and multidrug resistance TB (MDR TB). Ability of Mycobacterium tuberculosis (M. tuberculosis) to spread or to resist drugs varies from strain to strain and various strains have different geographical distributions however this information is inadequate in Ethiopia. So the objective of this study is to identify the species and strains of Mycobacteria isolates causing pulmonary TB, to assess drug susceptibility pattern and risk factors in and around Dessie, north east Ethiopia. Methods & Materials: A cross-sectional study was conducted between October 1 to September 30/2013 and Spoligotyping, RD9 deletion typing and proportion method drug susceptibility test using Middle Brook 7H10 was done. Results: A total of 92 smear positive pulmonary tuberculosis patients were enrolled. The mean age was 28.29 years. Children less than 18 years of age comprise 7.6% (n = 7). In this study 82.7% (n = 62) of the isolates were sensitive to all first line drugs. Resistance to any of the four first line drugs tested was 17.3% (n = 13). A high frequency resistance was observed in isonized, 12%, (n = 9). Resistance to any of the first line anti-tuberculosis drugs was not associated with HIV status, sex and history of previous TB treatment but it was significantly associated with age, high between 31-35 years of age (p = 0.025). No MDR TB identified. With respect to their Spoligotype pattern, the most frequent families were T family (T1, 36% (n = 27), T3, 10.7% (n = 8), CAS, 21.3% (n = 16), Haarlem3, 8% (n = 6), family 36, 8% (n = 6) and there was no Beijing family. Euro-American lineage comprised 48 (64%) but the lineage of 15 (20%) isolates were not found in the spotclust database, the remaining 12 (16%) were grouped into indo-oceanic lineage. Conclusion: High proportion resistance to any of the first line anti tuberculosis drugs was observed in this study. This may be a potential source in the emergence of MDR TB. The dominant strain circulating in the area belongs to the modern lineages which may indicate recent transmission.
Authors: M.M. Temesgen, S. Habtemariam, A. Aseffa, E. Gadisa
Institutions: Addis Ababa University, Armauer Hansen Research Institute
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,900
OpenAlex ID: W2052033737