Use of 23Na-Nuclear Magnetic Resonance To Follow Sodium Uptake and Efflux in NaCl-Adapted and Nonadapted Millet (Panicum miliaceum) Suspensions
Year: 1984
Type: article
Abstract: Cellular Na(+) transport was followed in vivo by (23)Na nuclear magnetic resonance (NMR) using anionic dysprosium-based shift reagents to resolve internal and external (23)Na(+) resonances. Proso millet (Panicum miliaceum) cell suspensions adapted for rapid growth on 130 mm NaCl had biphasic (23)Na efflux kinetics when shifted to low Na(+) medium, while nonadapted cells had little measurable Na(+) efflux after preloading with (23)NaCl. Uptake of (23)Na was also observed using (23)Na NMR. The resonance frequency of the external Na(+)-dysprosium (III) triphosphate, relative to that of the (23)Na in the cells, was sensitive to pH, permitting the pH of the external medium to be followed during the course of in vivo experiments.
Source: PLANT PHYSIOLOGY
DOI: 10.1104/pp.75.1.269
Authors: Laurel O. Sillerud, James W. Heyser
Institution: University of California System
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
Funder: U.S. Department of Energy
OpenAlex ID: W2053226283