Work

395: Impact of Donor T Cells on B Cell Development after Hematopoietic Cell Transplantation: Lessons from B Cell Deficient Mice

Year: 2008

Type: article

Abstract: Allogeneic hematopoietic cell transplant (HCT) recipients often exhibit B cell (BC) lymphopenia due, in part, to graft-versus-host-disease (GVHD). Here, we studied the influence of donor T cells (TC) on BC deficiency post-HCT. Following lethal irradiation, BALB. B mice were given FACS purified hematopoietic stem cells (HSC: cKIT+Thy1.1loLin-Sca-1+) alone, or with co-transfer of whole splenocytes (SP), CD4 or CD8 TCs from minor antigen-mismatched C57BL/6 (B6) mice. When pure HSC were transplanted, BC reconstituted promptly (median 33% of lymphocytes [d30]; 61% [d60]; 74% [d90]), whereas TC engraftment was retarded and did not achieve full donor chimerism.

Source: Transplantation and Cellular Therapy

DOI: 10.1016/j.bbmt.2007.12.405

Authors: A.M.S. Mueller, J.A. Allen, D. Miklos, J.W. Tung, J.A. Shizuru

Institutions: University Medical Center Freiburg, Stanford Medicine, Stanford University

Language: English

Topic: Hematopoietic Stem Cell Transplantation

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,650