Work

A Shotgun Proteomic Method for the Identification of Membrane-Embedded Proteins and Peptides

Year: 2008

Type: article

Abstract: Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome.

Source: Journal of Proteome Research

DOI: 10.1021/pr700795f

Authors: Adele R. Blackler, Anna E. Speers, Mark S. Ladinsky, Christine C. Wu

Institutions: University of Colorado Boulder, University of Colorado Anschutz

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institute on Alcohol Abuse and Alcoholism, National Institute on Drug Abuse, National Institute of Allergy and Infectious Diseases

Awards: G432172162, Proteomic Characterization of the Dopamine Transporter, Proteomic Tools for the Comprehensive Analysis of Dopamine Transporter Topology, G734774133