Sequence Analysis of Potato α-Amylase Gene amyA2
Year: 2013
Type: article
Abstract: The sequences of Potato α-Amylase Gene amyA2 was analysised by Bioinformatics, including its codon usage bias, physical and chemical properties, subcellular localization, and conserved structures. The results showed that the cDNA had a 1218 bp open reading frame and was referred to as amyA2, which encodes for an α-amylase with 405 amino acid residues (GenBank accession number: GU134783), and shared 98% identity with a published potato α-amylase (GenBank accession number: M79328.1) at the amino acid level. The amino sequences contains a catalytic domain (PF00128、SM00624) between 20 to 348 and a C-terminal beta-sheet domain between 349-407, which are similar to ones of the amylase family 13. Eight-stranded alpha/beta barrel was also found in the enzyme, which was thought as an active site of α-amylase.
Source: Advanced materials research
Authors: Yong Gang Wang, Jian Zhong Ma, Xue Qing Ma, Jin Ge Liu, Ming Jun Yang
Institution: Lanzhou University of Technology
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2053486401