Year: 2012
Type: erratum
Abstract: Mosaic Analysis with Double Markers (MADM) is a method for generating genetically mosaic mice, in which sibling mutant and wild-type cells are labeled with different fluorescent markers.It is a powerful tool that enables analysis of gene function at the single cell level in vivo.It requires transgenic cassettes to be located between the centromere and the mutation in the gene of interest on the same chromosome.Here we compare procedures for introduction of MADM cassettes into new loci in the mouse genome, and describe new approaches for expanding the utility of MADM.We show that: 1) Targeted homologous recombination outperforms random transgenesis in generation of reliably expressed MADM cassettes, 2) MADM cassettes in new genomic loci need to be validated for biallelic and ubiquitous expression, 3) Recombination between MADM cassettes on different chromosomes can be used to study reciprocal chromosomal deletions/duplications, and 4) MADM can be modified to permit transgene expression by combining it with a binary expression system.The advances described in this study expand current, and enable new and more versatile applications of MADM.
Source: PLoS ONE
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,382
Funders: National Science Foundation, Howard Hughes Medical Institute, Pew Charitable Trusts, Damon Runyon Cancer Research Foundation, European Molecular Biology Organization, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, National Institutes of Health, Japan Society for the Promotion of Science, National Institute of Neurological Disorders and Stroke
OpenAlex ID: W2053544067