Work

Telomeres of polytene chromosomes in a ciliated protozoan terminate in duplex DNA loops

Year: 1999

Type: article

Abstract: The end of a telomeric DNA sequence isolated from a polytene chromosome of a hypotrichous ciliate folds back and hybridizes with downstream telomeric sequence to form a t loop that is stable in the absence of protein and DNA cross-linking. The single-stranded, telomeric DNA sequence at the end of a macronuclear molecule does not form a t loop but, instead, is complexed with a heterodimeric, telomere-binding protein. Thus, two mechanisms for capping the ends of DNA molecules are used in the same cell.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.96.25.14436

Authors: K. G. Murti, D. M. Prescott

Institutions: St. Jude Children's Research Hospital, University of Colorado Boulder

Language: English

Topic: Telomeres, Telomerase, and Senescence

Subfield: Physiology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTION, G609259107, G7573229021, G9000086855, Cancer Center Support Grant (CCSG)