Work

Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge

Year: 2007

Type: article

Abstract: This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.

Source: The Open Analytical Chemistry Journal

DOI: 10.2174/1874065000701010028

Authors: Yoichi Shibusawa, Yoichiro Ito

Institutions: Tokyo University of Pharmacy and Life Sciences, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chemical and Physical Properties in Aqueous Solutions

Subfield: Filtration and Separation

Field: Chemical Engineering

Domain: Physical Sciences

Open Access status: bronze

Funder: National Institutes of Health

Award: G4705346009