Isolation and Purification of Psoralen and Bergapten from Ficus carica L. Leaves by High-Speed Countercurrent Chromatography
Year: 2008
Type: article
Abstract: Chi Chunyana, Shi Boa, Liang Pinga, Li Jingmeib & Yoichiro Itoc* a Feed Research Institute, Chinese Academy of Agricultural Sciences , Beijing, P. R. China b Cereal Quality Supervision and Testing Center of Ministry of Agriculture , Beijing, P. R. China c Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health , Bethesda, Maryland, USA * Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bldg. 10, Room 8N230, Rockville Pike, Bethesda, MD 20892–8014 E-mail: [email protected] Abstract High-speed countercurrent chromatography (HSCCC) was successfully applied for the first time for the isolation and purification of psoralen and bergapten from the plant Ficus carica L leaves. The crude extract was obtained by extraction with light petroleum (b.p.: 60°C–90°C) from the dried leaves of Ficus carica L. n-Hexane-ethyl acetate-methanol-water (1:1:1:1, v/v) was used as the two-phase solvent system. Each peak fraction was analyzed by high performance liquid chromatography (HPLC). The method yielded 4.4 mg of psoralen at 99.1% purity and 2.1 mg of bergapten at 98.2% purity from 400 mg of the crude extract in a single run. The structures of the two compounds were identified by 1H-NMR, 13C-NMR, and MS.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funder: National Institutes of Health
Award: G4705346009
OpenAlex ID: W2054357754