Work

High Resolution Phase-Sensitive Magnetomotive Optical Coherence Microscopy for Tracking Magnetic Microbeads and Cellular Mechanics

Year: 2013

Type: article

Abstract: We present a real-time multimodal near-infrared imaging technology that tracks externally induced axial motion of magnetic microbeads in single cells in culture. The integrated multimodal imaging technique consists of phase-sensitive magnetomotive optical coherence microscopy (MM-OCM) and multiphoton microscopy (MPM).MPMis utilized for the visualization of multifunctional fluorescent and magnetic microbeads, while MM-OCM detects, with nanometer-scale sensitivity, periodic displacements of the microbeads induced by the modulation of an external magnetic field. Magnetomotive signals are measured from mouse macrophages, human breast primary ductal carcinoma cells, and human breast epithelial cells in culture, and validated with full-field phase-sensitive microscopy. This methodology demonstrates the capability for imaging controlled cell dynamics and has the potential for measuring cell biomechanical properties, which are important in assessing the health and pathological state of cells.

Source: IEEE Journal of Selected Topics in Quantum Electronics

DOI: 10.1109/jstqe.2013.2280501

Authors: Vasilica Crecea, Benedikt W. Graf, Taewoo Kim, Gabriel Popescu, Stephen A. Boppart

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Optical Coherence Tomography Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Institute of Environmental Health Sciences, National Institute of Biomedical Imaging and Bioengineering

Awards: G299089034, Targeted Microspheres for Contrast Enhancement in Optical Coherence Tomography