Work

Clone‐specific anti‐CD45 blocking factor in patient plasma

Year: 2007

Type: article

Abstract: We observed inhibition of anti-CD45, but not anti-CD3, -CD4, or -CD8 staining in the whole blood of an individual clinical specimen. Subsequent experiments demonstrated the presence of a plasma factor, which likewise blocked CD45 staining of third party lymphocytes. The blocking activity was anti-CD45 clone (2D1) specific. Experiments utilizing immunoabsorption, immunofluorescence, and commercial blocking reagents failed to provide evidence for identifying blocking factor as heterophilic antibody. Biochemical identification of blocking factor was not accomplished.

Source: Cytometry Part B Clinical Cytometry

DOI: 10.1002/cyto.b.20168

Authors: Samuel R. Wellhausen, Stephen P. Slone, James J. Miller

Institution: University of Louisville

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: University of Louisville