Application of Preparative High-Speed Countercurrent Chromatography for Separation of Elatine fromDelphinium shawurense
Year: 2008
Type: article
Abstract: Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high-speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800 rpm. This yielded 72 mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funder: National Institutes of Health
Award: G4705346009
OpenAlex ID: W2055000570