Functional Mechanotransduction Is Required for Cisplatin-Induced Hair Cell Death in the Zebrafish Lateral Line
Year: 2013
Type: article
Abstract: Cisplatin, one of the most commonly used anticancer drugs, is known to cause inner ear hair cell damage and hearing loss. Despite much investigation into mechanisms of cisplatin-induced hair cell death, little is known about the mechanism whereby cisplatin is selectively toxic to hair cells. Using hair cells of the zebrafish lateral line, we found that chemical inhibition of mechanotransduction with quinine and EGTA protected against cisplatin-induced hair cell death. Furthermore, we found that the zebrafish mutants mariner (myo7aa) and sputnik (cad23) that lack functional mechanotransduction were resistant to cisplatin-induced hair cell death. Using a fluorescent analog of cisplatin, we found that chemical or genetic inhibition of mechanotransduction prevented its uptake. These findings demonstrate that cisplatin-induced hair cell death is dependent on functional mechanotransduction in the zebrafish lateral line.
Source: Journal of Neuroscience
Language: English
Subfield: Sensory Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Awards: G228583120, Intelligent Imaging Spinning Disk Confocal Microscope, Chemoprotection Against Aminoglycoside-Induced Ototoxicity, G3956554901, Identifying and charecterizing chemical modulators of hair cell death, G5024083513, Research Training in Otolaryngology, Intercellular trafficking of labeled aminoglycosides in living hair cells, Core Center - NIDCD Research Core Center P30, Auditory Neuroscience Training Program
OpenAlex ID: W2055368684