Year: 2010
Type: review
Abstract: Fluorescence microscopy is one of the most powerful tools to investigate complex cellular processes such as cell division, cell motility, or intracellular trafficking. The availability of RNA interference (RNAi) technology and automated microscopy has opened the possibility to perform cellular imaging in functional genomics and other large-scale applications. Although imaging often dramatically increases the content of a screening assay, it poses new challenges to achieve accurate quantitative annotation and therefore needs to be carefully adjusted to the specific needs of individual screening applications. In this review, we discuss principles of assay design, large-scale RNAi, microscope automation, and computational data analysis. We highlight strategies for imaging-based RNAi screening adapted to different library and assay designs.
Source: The Journal of Cell Biology
Authors: Christian Conrad, Daniel W. Gerlich
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2056022845