Work

PX DNA Triangle Oligomerized Using a Novel Three-Domain Motif

Year: 2007

Type: article

Abstract: Structural DNA nanotechnology is directed at building objects, lattices, and arrays from cohesive interactions between DNA molecules. The predominant means of doing this takes advantage of the information inherent in Watson-Crick base pairing in duplex formation and in sticky-ended cohesion. Nevertheless, other forms of nucleic acid cohesion are also known, particularly paranemic edge-sharing interactions (PX). Here we report the formation of a triangular species that has four strands per edge, held together by PX interactions. We demonstrate by nondenaturing gel electrophoresis and by atomic force microscopy (AFM) that we can combine a partial triangle with other strands to form a four-stranded molecule that is robust. By combining them with a new mixed-fusion type of three-domain molecule, we demonstrate by AFM that these triangles can be self-assembled into a linear array.

Source: Nano Letters

DOI: 10.1021/nl072803r

Authors: Wenyan Liu, Xing Wang, Tong Wang, Ruojie Sha, Nadrian C. Seeman

Institution: New York University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Physical Chemistry of Recombinational intermediates, G5356741539, Physical Chemistry of Recombinational Intermediates