Work

Enhanced non-viral gene delivery to human embryonic stem cells via small molecule-mediated transient alteration of the cell structure

Year: 2014

Type: article

Abstract: -(4-pyridyl)cyclohexanecarboxamide (Y-27632), a small molecule that inhibits the rho-associated protein kinase pathway, is utilized to induce transient colony spreading which leads to increased transfection efficiency by 1.5 to 2 folds in a spectrum of non-viral transfection reagents including Lipofectamine 2000 and Fugene HD. After removal of Y-27632 post-transfection, cells can revert back to its normal state and do not show alteration of pluripotency. This approach provides a simple, effective tool to enhance non-viral gene delivery into adherent hESCs for genetic manipulation.

Source: Journal of Materials Chemistry B

DOI: 10.1039/c4tb00750f

Authors: Jonathan Yen, Lichen Yin, Jianjun Cheng

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, University of Illinois at Urbana-Champaign, National Institute of Biomedical Imaging and Bioengineering